Regulatory
Part:BBa_K1592022
Designed by: Shuyan Tang Group: iGEM15_HUST-China (2015-09-09)
ptrp mutant3
Ptrp(BBa_K191007) is a promoter with the trp operator. It will be repressed by trpR (BBa_K588000) and LovTAP.
Note that the original trp operator contains two SpeI sites.
We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1(BBa_K1592020), ptrp mutant2(BBa_K1592021), and ptrp mutant3(BBa_K1592022).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |